LOCUS       sequence-102319-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    63..180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    229..796
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    247..798
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(391..408)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     618..637
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(846..867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg gtatcggtta
       61 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
      121 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
      181 atcgatcacg agactagcct cgaggtttaa actacgggct gcaggaattc cgcccccccc
      241 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
      301 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
      361 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
      421 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
      481 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
      541 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
      601 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
      661 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
      721 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
      781 aaaaacacga tgataatacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      841 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      901 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      961 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgctt
//
