LOCUS       sequence-102323-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luminescent reporter of insulin secretion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Burns SM, Vetere A, Walpita D, Dancik V, Khodier C, Perez J, Clemons
            PA, Wagner BK, Altshuler D
  TITLE     High-throughput luminescent reporter of insulin secretion for 
            discovering regulators of pancreatic Beta-cell function.
  JOURNAL   Cell Metab. 2015 Jan 6;21(1):126-37. doi: 
            10.1016/j.cmet.2014.12.010.
  PUBMED    25565210
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.12"; journalName: "Cell Metab"; date: 
            "2015-01-6- 6"; volume: "21"; issue: "1"; pages: "126-37"
COMMENT     Sequence Label: Proinsulin-NanoLuc in pLX304
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..38
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actgtcggga tcaacaagtt tgtacaaaaa agcaggctta atggccctgt ggatgcgctt
       61 cctgcccctg ctggccctgc tcttcctctg ggagtcccac cccacccagg cttttgtcaa
      121 gcagcacctt tgtggttccc acctggtgga ggctctctac ctggtgtgtg gggagcgtgg
      181 cttcttctac acacccatgt cccgccgtga agtggaggac ccacaagtgg cacaactgga
      241 gctgggtgga ggcccgggag caggtgacct tcagaccttg gcactggagg tggcccagca
      301 gaagagggtc ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa
      361 cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc
      421 cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca
      481 tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt
      541 taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact
      601 ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat
      661 cgccgtgttc gacggcaaaa agatcactgt aa
//
