LOCUS       sequence-102324-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luminescent reporter of insulin secretion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Burns SM, Vetere A, Walpita D, Dancik V, Khodier C, Perez J, Clemons
            PA, Wagner BK, Altshuler D
  TITLE     High-throughput luminescent reporter of insulin secretion for 
            discovering regulators of pancreatic Beta-cell function.
  JOURNAL   Cell Metab. 2015 Jan 6;21(1):126-37. doi: 
            10.1016/j.cmet.2014.12.010.
  PUBMED    25565210
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.12"; journalName: "Cell Metab"; date: 
            "2015-01-6- 6"; volume: "21"; issue: "1"; pages: "126-37"
COMMENT     Sequence Label: Proinsulin-NanoLuc in pLX304
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..91)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(191..700)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
ORIGIN
        1 ggttgattgt cgacttaacg cgccaccggt tagcgctagc tcattactac gtagaatcga
       61 gaccgaggag agggttaggg ataggcttac caaccacttt gtacaagaaa gctgggtact
      121 agttgcagta gttctccagc tggtagaggg agcagatgct ggtgcagcac tgatctacaa
      181 tgccacgctt cgccagaatg cgttcgcaca gccgccagcc ggtcactccg ttgatggtta
      241 ctcggaacag cagggagccg tcggggttga tcaggcgctc gtcgataatt ttgttgccgt
      301 tccacagggt ccctgttaca gtgatctttt tgccgtcgaa cacggcgatg ccttcatacg
      361 gccgtccgaa atagtcgatc atgttcggcg taaccccgtc gattaccagt gtgccatagt
      421 gcaggatcac cttaaagtga tgatcatcca cagggtacac caccttaaaa attttttcga
      481 tctggcccat ttggtcgccg ctcagacctt catacgggat gatgacatgg atgtcgatct
      541 tcagcccatt ttcaccgctc aggacaatcc tttggatcgg agttacggac accccgagat
      601 tctgaaacaa actggacaca cctccctgtt caaggacttg gtccaggttg tagccggctg
      661 tctgtcgcca gtccccaacg aaatcttcga gtgtgaagac cctcttctgc tgggccacct
      721 ccagtgccaa ggtctgaa
//
