LOCUS       sequence-102339-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His tagged Drosophila musashi RRM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     musashi RBD protein expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Dm_msi_RRM1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(67..114)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     110..128
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(181..198)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(804..824)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggcggcttaa tgcgccgcta cagggcgcgt cccattcgcc aatccggata tagttcctcc
       61 tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctagttattg
      121 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatctca
      181 gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg tcgacttcta cggtgtgact
      241 gcttccttgg gctgtgcctt cttgcactcg accttcttgt tcttgatggt gtggaagtga
      301 atctcacata cgcgatcgac cacatcctca ttctcaaagg tgacaaatcc gaagccacgg
      361 tggcgctttg tctgctggtc catcaacata accgtctcct cgacgggtcc gaactggctg
      421 aagtaagcct taacctcctc ggccgaagta tcctgtgaga cgccgcccac gaagatcttc
      481 ttggtcttgt tggcctaacg aggtcgattc tttggggtgg catgcttggg atcgatcttc
      541 ttgccatcga gtgtgtgaat gggcaccttg agaaccttct ccacggtgca gggttcttgg
      601 aatgtgatga aaccaaagcc gcgactgcgc tgggtgacgg gatccttcat gatgagcaca
      661 tcggtgacgg tgccgaacat gttgaagtat tccttgagct tgtcagacga cgtctgccag
      721 ctgagaccgc cgacaaagag tttgccgggt gccggatcgg agccactgcc tccgctcagg
      781 ctggggagct cgaattcgga tccgccctga aaatacaggt tttcgccgtg atgatg
//
