LOCUS       sequence-102341-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing TRP1 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5608_TEF1*-Cre_TRP1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(5..194)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     206..224
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
       61 acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta
      121 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt
      181 cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg tgacataact aattacatga
      241 ctcgaggtcg acaattccaa ccttacccaa gagttcgcca aactcagaca tcactttagc
      301 aaaaccgcgc cgtgcttctt cctcggtggc attcatcacg aaatgttcag cactacgcat
      361 acttttgaca ggaaacgcaa cggatattga gtcaatatca ggcattctat cgctcagctt
      421 tacagtgaca atgacggctg gcgactgaat attagtgctt acagacagca ctacatattt
      481 tccgtcgatg ttgaaatcct ttctcatatg tcaccataaa tatcaaataa ttatagcaat
      541 catttacgcg ttaatggcta atcgccatct tccagcaggc gcaccattgc ccctgtttca
      601 ctatccaggt tacggatata gttcatgaca atatttacat tggtccagcc accagcttgc
      661 atgatctccg gtattgaaac tccagcgcgg gccatatctc gcgcggctcc gacacgggca
      721 ctgtgtccag accaggccag gtatctctga ccagagtcat ccttagcgcc gtaaatcaat
      781 cgatgagttg cttcaaaaat cccttccagg gcgcgagttg atagctggct ggtggcagat
      841 ggcgcggcaa caccattttt t
//
