LOCUS       sequence-102342-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His tagged human MSI2 RNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     musashi RBD protein expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Hs_MSI2_RBD
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(52..99)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     95..113
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(166..183)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca gcaaaaaacc
       61 cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag cggtggcagc
      121 agccaactca gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg
      181 gtgctcgagt gcggccgcaa gctttcatgg gaacatgact tctttcggct gagctttctt
      241 acattctacc attttattat tgatttcatg gaaatgaatc tcacagactt tctccacaac
      301 atcttcattc tcaaaagtga caaagccaaa ccctctgtgc ctgttggtag ttttatcaaa
      361 catcagcatt gcatcttcca ccttgccaaa ctgctcgaaa tattgcttta catcttccac
      421 tactgtgttc gcagataacc cgcctacaaa tattttcttt gttctcgtga ccatcttggg
      481 ttgcgctcga cgaggaaatg caactttggg gtcaatcgtc ttggaatcta actcatggtg
      541 gggctgacct aatactttat ctacacttgc tgggtctgcg aacgtgacga aaccgaagcc
      601 tctggagcgt ttcgtagtgg gatctctcat gaccatacat tctctaattt ctccaaattt
      661 gctaaaatag tctctaaggc tatctggtga ggtctgccag ctcagtccac cgataaacat
      721 tttaccgggg tcgtgctggg agtcgttggc gctgcccgag gtgccggatc cgccctgaaa
      781 ata
//
