LOCUS       sequence-102344-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing TRP1 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5608_TEF1*-Cre_TRP1
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     228..248
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        230..248
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
      241 actaaaggga acaaaagctg gatagcttca aaatgtctct actccttttt tactcttcca
      301 gattttctcg gactccgcgc accgccgtac cacttcaaaa cacccaagca cagcatacta
      361 aattccccct cctccttcct ctagggtgcc gttaattacc cgtactaaag gtttggaaaa
      421 ggaaaaagag accgcctcgt ccctttttct tcgtcggaga aggcaataaa aatttttatc
      481 acgtttcttt ctcttgaaaa cttttttttc gattttgttc tctttcgacg acctcccatt
      541 gatatttgag ttaacaaacg gtcttcaatt tctcaagttt cagcttcatt tttcctgttc
      601 tattacaact ttttttactt cttgctcatt ggaaagaaag catagcaatc taatctaagt
      661 tttaattaca aaaactagtg gatcccccgg gctgcaggaa ttcgatatca agcttatcga
      721 taccgtcgag gggcagagcc gatcctgtac actttactta aaaccattat ctgagtgtta
      781 aatgtccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag
      841 tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca
      901 tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa
      961 ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc
     1021 gcgcggtctg g
//
