LOCUS       sequence-102349-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent Cas9 expression plasmid and targeting vector for the 
            mouse Rosa26 locus. Cas9 expression is Cre-dependent and the 
            targeting vector has positive (Neo) and negative (DTA) selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: LSL-Cas9-Rosa26TV
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..64
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    231..365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    485..619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gaacttcgtc gacatttaaa tcatttaaat ataacttcgt ataatgtatg ctatacgaag
       61 ttattcgcga tgaataaatg aaagcttgca gatctgcgac tctagaggat ctgcgactct
      121 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      181 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      361 ggatctgcga ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
      421 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
      481 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      601 atcttatcat gtctggatct gcgactctag aggatcataa tcag
//
