LOCUS       sequence-102383-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites 
            mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_d23
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag gtgagtactc
      121 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
      181 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
      241 aaagacaatc tttttgttgt caagctgttt gactcatgca cccgctactc ttcaaccttg
      301 cagactaatt aatggcaact ctgacagatg tggcgggagg aaagctgagg tgggagtact
      361 gctcagagct acaactctag gagtagggac tccaagccta gagctccaag agagggtgaa
      421 gggcagggag aaaattatag taatagttgc aatgagtcac ttgcttctag atcaagatca
      481 gagactctgc tctccgtgtt cacagcggac cttgatttaa tgtcatacaa ttaaggcacg
      541 cggtgaatgc caagagcgga gcctacggct gcacttgaag gacatccgag agaagttagg
      601 aagggtgggg agaaacaatt ctagaatgaa cccatcctgt gcgacacaag gacccctcgc
      661 attgattcaa aatctttaga atacaagaca gtcaaatgtt tttcagcgag aatctgggaa
      721 atccgcatgc atactcccaa ggggaatatt agaggtttca ttgacagctg tcaggagagc
      781 tgtagttccc
//
