LOCUS       sequence-102427-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains Plac driving expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PLac:GFP]
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     165..182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(217..303)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc
       61 gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc
      121 gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
      181 aggaagcgga atatatccct aggtctaggg cggcggattt gtcctactca ggagagcgtt
      241 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      301 ttgatgcctc tagcacgcgt ctagatcagc taattaagct ttcatttgta caattcatcc
      361 ataccatggg taataccagc agcagtaaca aattctaaca agaccatgtg gtctctcttt
      421 tcgtttggat ctttggataa tttagattga gtggataagt aatggttgtc tggtaacaag
      481 actggaccat caccaattgg agtattttgt tgataatggt cagctaattg aacagaacca
      541 tcttcaatgt tgtgtctaat tttgaagtta actttgatac cattcttttg tttgtcagcc
      601 atgatgtaaa cattgtgaga gttatagttg tattccaatt tgtgacctaa aatgttacca
      661 tcttctttaa aatcaatacc ttttaattcg attctattaa ctaaggtatc accttcaaac
      721 ttgacttcag ctct
//
