LOCUS       sequence-102432-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains Plac driving expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PLac:GFP]
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     200..218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    241..257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    264..280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             322..333
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga
       61 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
      121 cccaacctta ccagagggcg ccccagctgg caattccgac gtctaagaaa ccattattat
      181 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa
      241 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag
      301 gacgcactga ccgaattcat taaagaggag aaaggtacca tgtctaaagg tgaagaatta
      361 ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg tcacaaattt
      421 tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt aaaatttatt
      481 tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt aacttatggt
      541 gttcaatgtt tttctagata cccagatcat atgaaacaac atgacttttt caagtctgcc
      601 atgccagaag gttatgttca agaaagaact a
//
