LOCUS       sequence-102438-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PTAN driving expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PTAN:GFP]
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     206..224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             327..338
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
       61 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      121 gggcttccca accttaccag agggcgcccc agctggcaat tccgacgtct aagaaaccat
      181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct
      241 gagaatttta agcggcttaa aattgacatt ttaagcgctt aaaatgatac tgagcacatc
      301 agcaggacgc actgaccgaa ttcattaaag aggagaaagg taccatgtct aaaggtgaag
      361 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      421 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      481 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttaactt
      541 atggtgttca atgtttttct agatacccag atcatatgaa acaacatgac tttttcaagt
      601 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
      661 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
      721 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata
      781 actctcacaa tgtttacatc atggctgac
//
