LOCUS       sequence-102439-        9245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of Ngn1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams 
            D, Sanna PP, Baldwin KK
  TITLE     Selective conversion of fibroblasts into peripheral sensory neurons.
  JOURNAL   Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 
            Nov 24.
  PUBMED    25420069
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3887"; 
            journalName: "Nat Neurosci"; date: "2015-01"; volume: "18"; issue: 
            "1"; pages: "25-35"
COMMENT     Sequence Label: Teto Ngn1
FEATURES             Location/Qualifiers
     source          1..9245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2599..2617,2618..2640,2641..2659,2660..2682,2683..2701,
                     2702..2724,2725..2743,2744..2766,2767..2785,2786..2808,
                     2809..2827,2828..2850,2851..2869)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2902..2940
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2937..2961
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3935..4523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3988..4008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4406..4417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4526..4542)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4527..4543)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5048..5228
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5254..5271)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5260..5484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5530..5958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5617..5636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5827..5848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5967..5987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5972..6301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6152..6287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6214..6233
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6349..6396
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6415..6789
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6919..7040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6956..6975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7010..7029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7089..7105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7089..7105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7102..7124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7113..7129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7137..7143,7144..7161,7162..7167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7182..7203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7320..7337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7491..8079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7571..7590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8250..9041,9042..9110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8873..8892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9111..9215)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaaaggcg tatcacgagg ccctttcgtc
     2581 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2641 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2701 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2761 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2821 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2881 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
     2941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     3001 agacaccggg accgatccag cctcctgcag gtctagagcc accatgcctg cccctttgga
     3061 gacctgcatc tctgatctcg actgctccag cagcaacagc agcagcgacc tgtccagctt
     3121 cctcaccgac gaggaggact gtgccaggct acagccccta gcctccacct cggggctgtc
     3181 cgtgccagcc cggaggagcg ctcccgccct ctccggggca tcgaatgttc ccggtgccca
     3241 ggacgaagag caggaacggc ggaggcggcg aggtcgcgct cgggtgcggt ccgaggctct
     3301 gctgcactcc ctgcggagga gtcgtcgcgt caaagccaac gatcgcgagc gcaaccgcat
     3361 gcacaacctc aacgctgcgc tggacgcctt gcgcagcgtg ctgccctcgt tccccgacga
     3421 caccaagctc accaagattg agacgctgcg cttcgcctac aactacatct gggccctggc
     3481 tgagacactg cgcctggcag atcaagggct ccccgggggc agtgcccggg agcgcctcct
     3541 gcctccgcag tgtgtcccct gtctgcccgg gcccccgagc ccggccagcg acactgagtc
     3601 ctggggttcc ggggccgctg cctccccctg cgccactgtg gcatcaccac tctctgaccc
     3661 cagtagtccc tcggcttcag aagacttcac ctatggcccg ggcgatcccc ttttctcctt
     3721 tcctggcctg cccaaagacc tgctccacac gacgccctgt ttcatcccat accactaggc
     3781 ctttgtaagg caacatcaat actttcttcc tcccccagtc taagagcaat aatagatggg
     3841 gaactggctg aagcctccgg gggccacact tacccccaag tgaattcttc gaagctagcg
     3901 gatccgttaa caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3961 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4021 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4081 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4141 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4201 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4261 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4321 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4381 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4441 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4501 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     4561 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4621 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4681 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4741 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     4801 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     4861 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     4921 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     4981 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     5041 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5101 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5161 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5221 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5281 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5341 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5401 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5461 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5521 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5581 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5641 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5701 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5761 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5821 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5881 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5941 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6061 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6121 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6181 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6241 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6301 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     6361 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     6421 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     6481 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     6541 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     6601 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     6661 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     6721 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     6781 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6841 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6901 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7021 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7081 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7141 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7261 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7921 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8221 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9241 gacac
//
