LOCUS       sequence-102455-        1945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_chaining vector
FEATURES             Location/Qualifiers
     source          1..1945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(158..226,227..1018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(376..395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1189..1777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1678..1697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac ctcgagaacg tcgaccaatt cggggaaatg tgcgcggaac
       61 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      121 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      181 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      241 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      301 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      361 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      421 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      481 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      541 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      601 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      661 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
      721 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      781 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
      841 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
      901 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
      961 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1021 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1081 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1141 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1201 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1261 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1321 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1381 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1441 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1501 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1561 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1621 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1681 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1741 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1801 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1861 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1921 gaccgagcgc agcgagtcag tgagc
//
