LOCUS       sequence-102458-        1949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with MCS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_MCS3
FEATURES             Location/Qualifiers
     source          1..1949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(162..230,231..1022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(380..399)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1193..1781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1682..1701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgacgcgt aactcgagaa gcttaatcct gcaggtcgac aattcgggga aatgtgcgcg
       61 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1321 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1921 gaacgaccga gcgcagcgag tcagtgagc
//
