LOCUS       sequence-102459-        1946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with MCS4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_MCS4
FEATURES             Location/Qualifiers
     source          1..1946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(159..227,228..1019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(377..396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1190..1778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1679..1698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgacgcgt aactcgaggc agctgagatc tgtcgacaat tcggggaaat gtgcgcggaa
       61 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
      601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
      661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
      721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
      781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
      841 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
      901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
      961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1201 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1261 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1321 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1381 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1441 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1501 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1561 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1621 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1681 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1741 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1801 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1861 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1921 cgaccgagcg cagcgagtca gtgagc
//
