LOCUS       sequence-102461-        2628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_TagBFP
FEATURES             Location/Qualifiers
     source          1..2628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        736..840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(841..909,910..1701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1059..1078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1872..2460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2361..2380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagatgag cgagctgatt aaggagaaca tgcacatgaa
       61 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
      121 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
      181 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
      241 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
      301 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
      361 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
      421 gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg
      481 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
      541 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
      601 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga
      661 gcagcacgag gtggcagtgg ccagatacgc gggcctgggc ggcggcctga acgtcgacca
      721 attcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      781 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      841 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      901 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      961 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1021 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1081 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1141 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1201 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1261 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1321 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1381 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1441 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1501 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1561 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1621 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1681 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1741 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1801 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1861 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1921 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1981 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2041 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2101 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2161 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2221 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2281 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2341 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2401 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2461 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2521 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2581 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagc
//
