LOCUS       sequence-102463-        2634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with LSS-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_LSS-mKate2
FEATURES             Location/Qualifiers
     source          1..2634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        742..846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(847..915,916..1707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1065..1084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1878..2466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2367..2386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagatgag cgagctgatt aaggagaaca tgcacatgaa
       61 gctgtacatg gaaggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
      121 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctacc
      181 cttcgccttc gacatcttgg ctaccagctt catgtacggc agctacacct tcatcaacca
      241 cacccagggc atccccgact tctttaagca gtccttccct gagggcttca catgggagag
      301 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
      361 cggttgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
      421 gatgcagaag aaaacactcg gctgggaggc cggcaccgag atgctgtacc ccgctgacgg
      481 cggcctggaa ggcagatctg acgacgccct gaagctcgtg ggcgggggcc acctgatctg
      541 caacttgaag agcacataca gatccaagaa acccgctaag aatctcaagg tgcccggcgt
      601 ctactatgtg gaccgaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
      661 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaggt
      721 cgaccaattc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      841 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      901 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      961 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1021 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1081 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1141 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1201 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1261 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1321 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1381 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1441 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1501 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1561 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1621 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1681 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1741 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1801 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1861 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1921 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1981 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2041 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2101 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2161 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2221 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2281 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2341 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2401 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2461 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2521 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2581 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagc
//
