LOCUS       sequence-102468-        3369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_tdTomato
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..1453
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        1477..1581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1582..1650,1651..2442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1800..1819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2613..3201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3102..3121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagatggt gagcaagggc gaggacgtca tcaaagagtt
       61 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
      241 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
      361 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
      421 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
      481 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
      541 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
      601 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
      661 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
      721 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
      781 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      841 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      901 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      961 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1021 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1081 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     1141 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1201 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     1261 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     1321 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     1381 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     1441 cgagctgtac aaggtcgacc aattcgggga aatgtgcgcg gaacccctat ttgtttattt
     1501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2281 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3361 tcagtgagc
//
