LOCUS       sequence-102470-        2619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with mPlum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_mPlum
FEATURES             Location/Qualifiers
     source          1..2619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..703
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     427..446
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     621..644
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        727..831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(832..900,901..1692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1050..1069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1863..2451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2352..2371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagatggt gagcaagggc gaggaggtca tcaaggagtt
       61 catgcgcttc aaggagcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aggctgaagg tgaccaaggg
      181 tggccccctg cccttcgcct gggacatcct gtcccctcag atcatgtacg gctccaaggc
      241 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      361 ctccctgcag gacggcgagt tcatctacaa ggtgaaggtg cgcggcacca acttcccctc
      421 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      481 ccccgaggac ggcgccctga agggcgagat gaagatgagg ctgaggctga aggacggcgg
      541 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
      601 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
      661 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gccgtcgacc aattcgggga
      721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1021 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1081 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1141 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1201 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1261 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1321 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1381 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1441 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1501 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1561 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1621 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1681 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1741 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1801 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1861 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1921 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1981 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2041 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2101 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2161 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2221 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2281 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2341 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2401 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2461 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2521 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2581 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagc
//
