LOCUS       sequence-102471-        2616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with E2-Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_E2-Crimson
FEATURES             Location/Qualifiers
     source          1..2616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..700
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     424..443
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        724..828
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(829..897,898..1689)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1047..1066)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1860..2448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2349..2368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagatgga tagcactgag aacgtcatca agcccttcat
       61 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcgt
      121 gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaagtga ccaagggcgg
      181 ccccctgccc ttcgcctggg acatcctgtc cccccagttc ttctacggct ccaaggcgta
      241 catcaagcac cccgccgaca tccccgacta cctcaagcag tccttccccg agggcttcaa
      301 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      361 cctgcaggac ggcaccctca tctaccacgt gaagttcatc ggcgtgaact tcccctccga
      421 cggccccgta atgcagaaga agactctggg ctgggagccc tccactgagc gcaactaccc
      481 ccgcgacggc gtgctgaagg gcgagaacca catggcgctg aagctgaagg gcggcggcca
      541 ctacctgtgt gagttcaagt ccatctacat ggccaagaag cccgtgaagc tgcccggcta
      601 ccactacgtg gactacaagc tcgacatcac ctcccacaac gaggactaca ccgtggtgga
      661 gcagtacgag cgcgccgagg cccgccacca cctgttccag gtcgaccaat tcggggaaat
      721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      841 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      901 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      961 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1021 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1081 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1141 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1201 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1261 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1321 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1381 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1441 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1501 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1561 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1621 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1681 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1741 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2161 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2221 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2281 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2341 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2401 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2461 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2521 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2581 cgcagccgaa cgaccgagcg cagcgagtca gtgagc
//
