LOCUS       sequence-102472-        2880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with iRFP670.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_iRFP670
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..958
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     promoter        988..1092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1093..1161,1162..1953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1311..1330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2124..2712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2613..2632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagatggc gcgtaaggtc gatctcacct cctgcgatcg
       61 cgagccgatc cacatccccg gcagcattca gccgtgcggc tgcctgctag cctgcgacgc
      121 gcaggcggtg cggatcacgc gcattacgga aaatgccggc gcgttctttg gacgcgaaac
      181 tccgcgggtc ggtgagctac tcgccgatta cttcggcgag accgaagccc atgcgctgcg
      241 caacgcactg gcgcagtcct ccgatccaaa gcgaccggcg ctgatcttcg gttggcgcga
      301 cggcctgacc ggccgcacct tcgacatctc actgcatcgc catgacggta catcgatcat
      361 cgagttcgag cctgcggcgg ccgaacaggc cgacaatccg ctgcggctga cgcggcagat
      421 catcgcgcgc accaaagaac tgaagtcgct cgaagagatg gccgcacggg tgccgcgcta
      481 tctgcaggcg atgctcggct atcaccgcgt gatgttgtac cgcttcgcgg acgacggctc
      541 cgggatggtg atcggcgagg cgaagcgcag cgacctggag agctttctcg gtcagcactt
      601 tccggcgtcg ctggtcccgc agcaggcgcg gctactgtac ttgaagaacg cgatccgcgt
      661 ggtctcggat tcgcgcggca tcagcagccg gatcgtgccc gagcacgacg cctccggcgc
      721 cgcgctcgat ctgtcgttcg cgcacctgcg cagcatctcg ccctgccatc tcgaatttct
      781 gcggaacatg ggcgtcagcg cctcgatgtc gctgtcgatc atcattgacg gcacgctatg
      841 gggattgatc atctgtcatc attacgagcc gcgtgccgtg ccgatggcgc agcgcgtcgc
      901 ggccgaaatg ttcgccgact tcttatcgct gcacttcacc gccgcccacc accaacgcgg
      961 atccgtcgac caattcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2821 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
//
