LOCUS       sequence-102475-        2449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK
FEATURES             Location/Qualifiers
     source          1..2449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..531
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(67..90)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     464..483
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        557..661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(662..730,731..1522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(880..899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1693..2281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2182..2201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtctac cgggtagggg aggcgctttt cccaaggcag
       61 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      121 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      181 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      241 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      301 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      361 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      421 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
      481 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacgtcgacc
      541 aattcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      601 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      661 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      721 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      781 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      841 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      901 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      961 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1021 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1081 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1141 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1201 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1261 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1321 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1381 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1441 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1501 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1561 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2341 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2401 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagc
//
