LOCUS       sequence-102480-        2323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with CMVenhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_CMVenhancer
FEATURES             Location/Qualifiers
     source          1..2323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        31..410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        431..535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(536..604,605..1396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(754..773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1567..2155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2056..2075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac ctcgagaacc catggtaata gcgatgacta atacgtagat
       61 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      121 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      181 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      241 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      301 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      361 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc gaccaattcg
      421 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      481 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      541 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      601 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      661 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      721 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      781 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      841 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      901 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      961 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1021 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1081 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1141 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1201 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1261 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1321 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1381 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1441 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1501 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1561 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1621 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1681 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1741 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1801 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1861 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1921 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2101 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2161 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2221 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2281 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agc
//
