LOCUS       sequence-102485-        3127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with bGpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_bGpA
FEATURES             Location/Qualifiers
     source          1..3127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          56..628
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(760..779)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    825..880
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(879..898)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        1235..1339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1340..1408,1409..2200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1558..1577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2371..2959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2860..2879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtgaat gaatgactag actgagaact tcagggtgag
       61 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
      121 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
      181 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
      241 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
      301 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatctc ttttttttca
      361 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
      421 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
      481 tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt tacaatgata
      541 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
      601 tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
      661 tcatcatttt ggcaaagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
      721 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
      781 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
      841 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
      901 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
      961 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     1021 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     1081 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     1141 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     1201 aactcgactg cgtcgaccaa ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1561 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1861 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2281 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3121 agtgagc
//
