LOCUS       sequence-102487-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with SV40pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_SV40pA
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    149..230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(186..205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        278..382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(383..451,452..1243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(601..620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1414..2002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1903..1922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtgaat gaatgaccag aggatcataa tcagccatac
       61 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      121 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc cccgagcttc
      241 ctcgctcact gactgtcgac caattcgggg aaatgtgcgc ggaaccccta tttgtttatt
      301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      361 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      421 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      481 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      541 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      601 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      661 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      721 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      781 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      841 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      901 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      961 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1021 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1081 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1141 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1201 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2161 gtcagtgagc
//
