LOCUS       sequence-102490-        2696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with rTA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_tTA2
FEATURES             Location/Qualifiers
     source          1..2696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(34..651,652..657,658..693,694..696,697..732,733..735,
                     736..771,772..780)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        804..908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(909..977,978..1769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1127..1146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1940..2528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2429..2448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtcgcc accatgtcta gactggacaa gagcaaagtc
       61 ataaactctg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa
      121 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
      181 gccctgctcg atgccctggc aatcgagatg ctggacaggc atcataccca cttctgcccc
      241 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
      301 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
      361 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
      421 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggatcag
      481 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
      541 ctgagacaag caattgagct gttcgaccat cagggagccg aacctgcctt ccttttcggc
      601 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
      661 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
      721 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccggatga
      781 gtcgaccaat tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     1321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagc
//
