LOCUS       sequence-102491-        1977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with loxP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_loxP
FEATURES             Location/Qualifiers
     source          1..1977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    26..59
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        85..189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(190..258,259..1050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(408..427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1221..1809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1710..1729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagataac ttcgtataat gtatgctata cgaagttatg
       61 cgtcgaccaa ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      901 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1081 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1141 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1201 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1261 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1321 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1381 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1441 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1501 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1561 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1621 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1681 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1741 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1801 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1861 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1921 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagc
//
