LOCUS       sequence-102493-        2327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with H2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_H2B
FEATURES             Location/Qualifiers
     source          1..2327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             34..411
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        435..539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(540..608,609..1400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(758..777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1571..2159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2060..2079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtcgcc accatgccag agccagcgaa gtctgctccc
       61 gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag
      121 cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc
      181 caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac
      241 attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
      301 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
      361 cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa ggtcgaccaa
      421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1681 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2281 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagc
//
