LOCUS       sequence-102494-        2021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_P2A
FEATURES             Location/Qualifiers
     source          1..2021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             42..98
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        129..233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(234..302,303..1094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(452..471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1265..1853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1754..1773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac ctcgaggcta gcggcagcgg cgccacaaac ttctctctgc
       61 taaagcaagc aggtgatgtt gaagaaaacc ccgggcctgc atgcggtcga ccaattcggg
      121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1381 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag c
//
