LOCUS       sequence-102495-        2070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with PEST2D .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PEST2D
FEATURES             Location/Qualifiers
     source          1..2070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        178..282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(283..351,352..1143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(501..520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1314..1902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1803..1822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagaaact tagccatggc ttcccgccgg aggtggagga
       61 gcaggatgat ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg accgtcaccc
      121 tgccgcctgt gcttctgcta ggatcaatgt gtaggtcgac caattcgggg aaatgtgcgc
      181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2041 cgaacgaccg agcgcagcga gtcagtgagc
//
