LOCUS       sequence-102505-        4687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK::CreERT2-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK::CreERT2-bGHpA
FEATURES             Location/Qualifiers
     source          1..4687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..531
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(67..90)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     464..483
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             548..1576
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1589..2524
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1637..2530
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    2548..2772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2795..2899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2900..2968,2969..3760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3118..3137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3931..4519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4420..4439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtctac cgggtagggg aggcgctttt cccaaggcag
       61 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      121 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      181 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      241 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      301 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      361 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      421 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
      481 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacgtcgagt
      541 cgccaccatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
      601 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
      661 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
      721 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
      781 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
      841 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
      901 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
      961 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
     1021 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
     1081 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
     1141 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
     1201 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
     1261 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
     1321 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
     1381 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
     1441 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
     1501 aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
     1561 gctggaagat ggcgatctgg agccatctgc tggagacatg agagctgcca acctttggcc
     1621 aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga
     1681 ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc
     1741 taccagaccc ttcagtgaag cctcgatgat gggcttactg accaacctgg cagacaggga
     1801 gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca
     1861 tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg
     1921 gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa
     1981 ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc
     2041 tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt
     2101 gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga
     2161 ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc
     2221 aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca
     2281 catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt
     2341 ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac
     2401 tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc
     2461 tacttcatcg cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc
     2521 cacagcttga gtcgagtgaa tgaatgactg tgccttctag ttgccagcca tctgttgttt
     2581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2761 tgggctctat ggtcgaccaa ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2821 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2881 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2941 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3001 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3061 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3121 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3181 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3241 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3301 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3361 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3421 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3481 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3541 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3601 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3661 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3721 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3781 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3841 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3901 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3961 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4021 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4081 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4141 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4201 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4261 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4321 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4381 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4441 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4501 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4561 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4621 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4681 agtgagc
//
