LOCUS       sequence-102506-        3561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with CMV::rtTA3-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_CMV::rtTA3-bGHpA
FEATURES             Location/Qualifiers
     source          1..3561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(655..1272,1273..1278,1279..1314,1315..1317,1318..1353,
                     1354..1356,1357..1392,1393..1401)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1422..1646
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1669..1773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1774..1842,1843..2634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1992..2011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2805..3393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3294..3313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag ggatctcagc tggtcgaggc aggtcgaatt caccatgtct
      661 agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc
      721 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
      781 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
      841 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
      901 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
      961 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     1021 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     1081 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     1141 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
     1201 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     1261 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
     1321 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     1381 cttgacatgc tccccgggta actaagtaag gatctgtcga gctgtgcctt ctagttgcca
     1441 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1501 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1561 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1621 tgctggggat gcggtgggct ctatggtcga ccaattcggg gaaatgtgcg cggaacccct
     1681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1981 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3541 gagcgcagcg agtcagtgag c
//
