LOCUS       sequence-102510-       14696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with 6 cassettes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: ColorfulCell
FEATURES             Location/Qualifiers
     source          1..14696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(720..722,723..725,726..1436)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1455..1679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1745..2048
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2049..2252
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2298..2307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2349..2351,2352..3062)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3069..3071,3072..3782)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3813..3815,3816..4529)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    4536..4760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4826..5129
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5130..5333
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5379..5388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5391..5411
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7515..7739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7805..8108
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8109..8312
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             8439..9116
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQA"
     CDS             8439..9116
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQA"
     polyA_signal    9123..9347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9413..9716
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9717..9920
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             9966..10898
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     polyA_signal    10917..11141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11207..11510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11511..11714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             11825..12532
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11970..11988)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    12557..12781
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        12804..12908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12909..12977,12978..13769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13127..13146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13940..14528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14429..14448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag ggatctcagc tggtcgagat gaagtcgccg cccctcgtgc
      661 tggccgccct ggtggcctgc atcatcgtct tgggcttcaa ctactggatt gcggtcgaga
      721 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      781 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      841 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      901 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
      961 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1021 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1081 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1141 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1201 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1261 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1321 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1381 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagacag
     1441 atccatgagt cgagctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1501 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1561 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     1621 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     1681 cgagtagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1741 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1801 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1861 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1921 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1981 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2041 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     2101 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     2161 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     2221 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagg
     2281 gatctcagct ggtcgaggcc accatgggct gtatcaagag caagaggaag gacgtcgaga
     2341 acgtcgaggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2401 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2461 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2521 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
     2581 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2641 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2701 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2761 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
     2821 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2881 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2941 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     3001 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3061 aggtcgaggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3121 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3181 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3241 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
     3301 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3361 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3421 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3481 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
     3541 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3601 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3661 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     3721 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3781 aggtcgagag ctttgtgatc tctggggtcg aggtgagcaa gggcgaggag ctgttcaccg
     3841 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3901 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3961 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
     4021 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4081 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4141 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4201 tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg
     4261 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     4321 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4381 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     4441 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4501 ctctcggcat ggacgagctg tacaagtaag tcgagctgtg ccttctagtt gccagccatc
     4561 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4621 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4681 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4741 ggatgcggtg ggctctatgg tcgagtagtt attaatagta atcaattacg gggtcattag
     4801 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4861 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4921 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4981 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5041 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5101 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     5161 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5221 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     5281 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     5341 tgaaccgtca gatccgctag ggatctcagc tggtcgaggc caccatggct cctaagaaga
     5401 agaggaaggt ggtcgagagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     5461 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     5521 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     5581 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     5641 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     5701 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5761 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     5821 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     5881 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     5941 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     6001 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     6061 tggcagtggc cagatacgcg ggcctgggcg gcggcctgaa cgtcgagagc gagctgatta
     6121 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     6181 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     6241 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     6301 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     6361 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     6421 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     6481 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     6541 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     6601 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
     6661 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     6721 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatacgcg ggcctgggcg
     6781 gcggcctgaa cgtcgaggtg agctctatct ttggggtcga gagcgagctg attaaggaga
     6841 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
     6901 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     6961 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
     7021 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
     7081 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     7141 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
     7201 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
     7261 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
     7321 gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca
     7381 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
     7441 agacctacgt cgagcagcac gaggtggcag tggccagata cgcgggcctg ggcggcggcc
     7501 tgaactaagt cgagctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7561 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7621 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7681 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     7741 cgagtagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7801 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7861 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     7921 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     7981 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     8041 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     8101 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     8161 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     8221 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     8281 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagg
     8341 gatctcagct ggtcgagatg ctgagcctgc ggcagagcat ccggttcttc aagcccgcca
     8401 cccggaccct gtgcagcagc cggtacctgc tggtcgagat ggtgagcgtg atcaagcccg
     8461 agatgaagat caagctgtgc atgaggggca ccgtgaacgg ccacaacttc gtgatcgagg
     8521 gcgagggcaa gggcaacccc tacgagggca cccagatcct ggacctgaac gtgaccgagg
     8581 gcgcccccct gcccttcgcc tacgacatcc tgaccaccgt gttccagtac ggcaacaggg
     8641 ccttcaccaa gtaccccgcc gacatccagg actacttcaa gcagaccttc cccgagggct
     8701 accactggga gaggagcatg acctacgagg accagggcat ctgcaccgcc accagcaaca
     8761 tcagcatgag gggcgactgc ttcttctacg acatcaggtt cgacggcacc aacttccccc
     8821 ccaacggccc cgtgatgcag aagaagaccc tgaagtggga gcccagcacc gagaagatgt
     8881 acgtggagga cggcgtgctg aagggcgacg tgaacatgag gctgctgctg gagggcggcg
     8941 gccactacag gtgcgacttc aagaccacct acaaggccaa gaaggaggtg aggctgcccg
     9001 acgcccacaa gatcgaccac aggatcgaga tcctgaagca cgacaaggac tacaacaagg
     9061 tgaagctgta cgagaacgcc gtggccaggt actccatgct gcccagccag gcctaagtcg
     9121 agctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     9181 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     9241 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     9301 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggtcg agtagttatt
     9361 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     9421 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     9481 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     9541 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     9601 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     9661 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     9721 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     9781 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     9841 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     9901 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctaggga tctcagctgg
     9961 tcgagatggc gcgtaaggtc gatctcacct cctgcgatcg cgagccgatc cacatccccg
    10021 gcagcattca gccgtgcggc tgcctgctag cctgcgacgc gcaggcggtg cggatcacgc
    10081 gcattacgga aaatgccggc gcgttctttg gacgcgaaac tccgcgggtc ggtgagctac
    10141 tcgccgatta cttcggcgag accgaagccc atgcgctgcg caacgcactg gcgcagtcct
    10201 ccgatccaaa gcgaccggcg ctgatcttcg gttggcgcga cggcctgacc ggccgcacct
    10261 tcgacatctc actgcatcgc catgacggta catcgatcat cgagttcgag cctgcggcgg
    10321 ccgaacaggc cgacaatccg ctgcggctga cgcggcagat catcgcgcgc accaaagaac
    10381 tgaagtcgct cgaagagatg gccgcacggg tgccgcgcta tctgcaggcg atgctcggct
    10441 atcaccgcgt gatgttgtac cgcttcgcgg acgacggctc cgggatggtg atcggcgagg
    10501 cgaagcgcag cgacctggag agctttctcg gtcagcactt tccggcgtcg ctggtcccgc
    10561 agcaggcgcg gctactgtac ttgaagaacg cgatccgcgt ggtctcggat tcgcgcggca
    10621 tcagcagccg gatcgtgccc gagcacgacg cctccggcgc cgcgctcgat ctgtcgttcg
    10681 cgcacctgcg cagcatctcg ccctgccatc tcgaatttct gcggaacatg ggcgtcagcg
    10741 cctcgatgtc gctgtcgatc atcattgacg gcacgctatg gggattgatc atctgtcatc
    10801 attacgagcc gcgtgccgtg ccgatggcgc agcgcgtcgc ggccgaaatg ttcgccgact
    10861 tcttatcgct gcacttcacc gccgcccacc accaacgcag caagctgtga gtcgagctgt
    10921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    10981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    11041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    11101 agacaatagc aggcatgctg gggatgcggt gggctctatg gtcgagtagt tattaatagt
    11161 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    11221 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    11281 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    11341 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    11401 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    11461 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    11521 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    11581 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    11641 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    11701 atataagcag agctggttta gtgaaccgtc agatccgcta gggatctcag ctggtcgagt
    11761 cgccaccatg ctgctatccg tgccgttgct gctcggcctc ctcggcctgg ccgtcgccgt
    11821 cgagatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
    11881 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
    11941 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
    12001 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
    12061 gcaccccgcc gacattcccg actacttgaa gctgtccttc cccgagggct tcaagtggga
    12121 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
    12181 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
    12241 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
    12301 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
    12361 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
    12421 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
    12481 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtctaagga
    12541 cgagctgtga gtcgagctgt gccttctagt tgccagccat ctgttgtttg cccctccccc
    12601 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
    12661 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
    12721 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
    12781 gtcgaccaat tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    12841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    12901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    13021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    13081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    13141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    13201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    13261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    13321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    13381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    13441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    13501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    13561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    13621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    13681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    13741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    13801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    13861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    13981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    14041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    14101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    14161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    14221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    14281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    14341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    14401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    14461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    14521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    14581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    14641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagc
//
