LOCUS       sequence-102511-       16923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with 4 cassettes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16923)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 16923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: ChromosomesMembranesandCytoskeleton
FEATURES             Location/Qualifiers
     source          1..16923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             647..1024
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     polyA_signal    3128..3352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3418..3721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3722..3925
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3971..3980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4022..4024,4025..4735)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4742..4744,4745..5455)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5486..5488,5489..6202)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    6209..6433
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6499..6802
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6803..7006
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7054..7063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7060..7767
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             7783..8487
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             8503..9207
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     polyA_signal    10387..10611
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10677..10980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10981..11184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      11232..11241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11238..11240,11241..11243,11244..11954)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11961..11963,11964..12674)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(12681..12683,12684..13394)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    14784..15008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        15031..15135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15136..15204,15205..15996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15354..15373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16167..16755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16656..16675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag ggatctcagc tggtcgagtc gccaccatgc cagagccagc
      661 gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa
      721 aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt
      781 tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc
      841 gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa
      901 caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg
      961 ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc
     1021 taaggtcgag agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     1081 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     1141 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     1201 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     1261 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     1321 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     1381 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     1441 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     1501 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     1561 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     1621 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     1681 ggccagatac gcgggcctgg gcggcggcct gaacgtcgag agcgagctga ttaaggagaa
     1741 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     1801 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     1861 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     1921 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     1981 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     2041 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     2101 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     2161 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     2221 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
     2281 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     2341 gacctacgtc gagcagcacg aggtggcagt ggccagatac gcgggcctgg gcggcggcct
     2401 gaacgtcgag gtgagctcta tctttggggt cgagagcgag ctgattaagg agaacatgca
     2461 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg
     2521 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
     2581 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
     2641 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
     2701 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
     2761 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg
     2821 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
     2881 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
     2941 gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc
     3001 tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta
     3061 cgtcgagcag cacgaggtgg cagtggccag atacgcgggc ctgggcggcg gcctgaacta
     3121 agtcgagctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggtcgagtag
     3361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3541 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3901 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agggatctca
     3961 gctggtcgag gccaccatgg gctgtatcaa gagcaagagg aaggacgtcg agaacgtcga
     4021 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4081 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4141 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4201 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     4261 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4321 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4381 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4441 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
     4501 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4561 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4621 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4681 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggtcga
     4741 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4801 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4921 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     4981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     5041 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     5101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     5161 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
     5221 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     5281 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5341 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5401 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggtcga
     5461 gagctttgtg atctctgggg tcgaggtgag caagggcgag gagctgttca ccggggtggt
     5521 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5581 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5641 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc
     5701 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5761 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5821 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5881 ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca acgtctatat
     5941 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     6001 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6061 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     6121 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6181 catggacgag ctgtacaagt aagtcgagct gtgccttcta gttgccagcc atctgttgtt
     6241 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6301 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6361 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6421 gtgggctcta tggtcgagta gttattaata gtaatcaatt acggggtcat tagttcatag
     6481 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     6541 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     6601 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     6661 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     6721 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     6781 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     6841 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     6901 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     6961 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     7021 tcagatccgc tagggatctc agctggtcga gtcgccacca tggtgagcaa gggcgaggag
     7081 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     7141 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     7201 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     7261 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat tcccgactac
     7321 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     7381 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     7441 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     7501 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     7561 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     7621 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     7681 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     7741 accggcggca tggacgagct gtacaagtct acagatgtcg aggtgagcaa gggcgaggag
     7801 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     7861 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     7921 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     7981 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat tcccgactac
     8041 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     8101 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     8161 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     8221 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     8281 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     8341 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     8401 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     8461 accggcggca tggacgagct gtacaagtct acagatgtcg aggtgagcaa gggcgaggag
     8521 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     8581 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     8641 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     8701 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat tcccgactac
     8761 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     8821 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     8881 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     8941 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     9001 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     9061 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     9121 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     9181 accggcggca tggacgagct gtacaagtct acagatgtcg aggtgagctc tatctttggg
     9241 gtcgagatgg atgatgatat cgccgcgctc gtcgttgaca acggctccgg catgtgcaag
     9301 gccggcttcg cgggcgacga tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc
     9361 aggcaccagg gcgtgatggt gggcatgggt cagaaggatt cctatgtggg cgacgaggcc
     9421 cagagcaaga gaggcatcct caccctgaag taccccatcg agcacggcat cgtcaccaac
     9481 tgggacgaca tggagaaaat ctggcaccac accttctaca atgagctgcg tgtggctccc
     9541 gaggagcacc ccgtgctgct gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag
     9601 atgacccaga tcatgtttga gaccttcaac accccagcca tgtacgttgc tatccaggct
     9661 gtgctatccc tgtacgcctc tggccgtacc actggcatcg tgatggactc cggtgacggg
     9721 gtcacccaca ctgtgcccat ctacgagggg tatgccctcc cccatgccat cctgcgtctg
     9781 gacctggctg gccgggacct gactgactac ctcatgaaga tcctcaccga gcgcggctac
     9841 agcttcacca ccacggccga gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac
     9901 gtcgccctgg acttcgagca agagatggcc acggctgctt ccagctcctc cctggagaag
     9961 agctacgagc tgcctgacgg ccaggtcatc accattggca atgagcggtt ccgctgccct
    10021 gaggcactct tccagccttc cttcctgggc atggagtcct gtggcatcca cgaaactacc
    10081 ttcaactcca tcatgaagtg tgacgtggac atccgcaaag acctgtacgc caacacagtg
    10141 ctgtctggcg gcaccaccat gtaccctggc attgccgaca ggatgcagaa ggagatcact
    10201 gccctggcac ccagcacaat gaagatcaag atcattgctc ctcctgagcg caagtactcc
    10261 gtgtggatcg gcggctccat cctggcctcg ctgtccacct tccagcagat gtggatcagc
    10321 aagcaggagt atgacgagtc cggcccctcc atcgtccacc gcaaatgctt ctagagatct
    10381 gtcgagctgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10441 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10501 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10561 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gtcgagtagt
    10621 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    10681 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    10741 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    10801 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    10861 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    10921 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    10981 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
    11041 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
    11101 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
    11161 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gggatctcag
    11221 ctggtcgagt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
    11281 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
    11341 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    11401 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct
    11461 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
    11521 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
    11581 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
    11641 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
    11701 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
    11761 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
    11821 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
    11881 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
    11941 acgagctgta caaggtcgag gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
    12001 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
    12061 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    12121 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct
    12181 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
    12241 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
    12301 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
    12361 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
    12421 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
    12481 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
    12541 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
    12601 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
    12661 acgagctgta caaggtcgag gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
    12721 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
    12781 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    12841 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct
    12901 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
    12961 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
    13021 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
    13081 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
    13141 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
    13201 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
    13261 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
    13321 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
    13381 acgagctgta caaggtcgag agctttgtga tctctggggt cgagcgtgag tgcatctcca
    13441 tccacgttgg ccaggctggt gtccagattg gcaatgcctg ctgggagctc tactgcctgg
    13501 aacacggcat ccagcccgat ggccagatgc caagtgacaa gaccattggg ggaggagatg
    13561 actccttcaa caccttcttc agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt
    13621 ttgtagactt ggaacccaca gtcattgatg aagttcgcac tggcacctac cgccagctct
    13681 tccaccctga gcagctcatc acaggcaagg aagatgctgc caataactat gcccgagggc
    13741 actacaccat tggcaaggag atcattgacc ttgtgttgga ccgaattcgc aagctggctg
    13801 accagtgcac cggtcttcag ggcttcttgg ttttccacag ctttggtggg ggaactggtt
    13861 ctgggttcac ctccctgctc atggaacgtc tctcagttga ttatggcaag aagtccaagc
    13921 tggagttctc catttaccca gcaccccagg tttccacagc tgtagttgag ccctacaact
    13981 ccatcctcac cacccacacc accctggagc actctgattg tgccttcatg gtagacaatg
    14041 aggccatcta tgacatctgt cgtagaaacc tcgatatcga gcgcccaacc tacactaacc
    14101 ttaaccgcct tattagccag attgtgtcct ccatcactgc ttccctgaga tttgatggag
    14161 ccctgaatgt tgacctgaca gaattccaga ccaacctggt gccctacccc cgcatccact
    14221 tccctctggc cacatatgcc cctgtcatct ctgctgagaa agcctaccat gaacagcttt
    14281 ctgtagcaga gatcaccaat gcttgctttg agccagccaa ccagatggtg aaatgtgacc
    14341 ctcgccatgg taaatacatg gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag
    14401 atgtcaatgc tgccattgcc accatcaaaa ccaagcgcag catccagttt gtggattggt
    14461 gccccactgg cttcaaggtt ggcatcaact accagcctcc cactgtggtg cctggtggag
    14521 acctggccaa ggtacagaga gctgtgtgca tgctgagcaa caccacagcc attgctgagg
    14581 cctgggctcg cctggaccac aagtttgacc tgatgtatgc caagcgtgcc tttgttcact
    14641 ggtacgtggg tgaggggatg gaggaaggcg agttttcaga ggcccgtgaa gatatggctg
    14701 cccttgagaa ggattatgag gaggttggtg tggattctgt tgaaggagag ggtgaggaag
    14761 aaggagagga atactaagtc gagctgtgcc ttctagttgc cagccatctg ttgtttgccc
    14821 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    14881 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    14941 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    15001 ctctatggtc gaccaattcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    15061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    15121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    15181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    15241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    15301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    15361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    15421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    15481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    15541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    15601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    15661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    15721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    15781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    15841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    15901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    15961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    16021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    16081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    16141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    16201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    16261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    16321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    16381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    16441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    16501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    16561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    16621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    16681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    16741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    16801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    16861 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    16921 agc
//
