LOCUS       sequence-102518-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient overexpression of Flag-Sesn1b (mouse).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Peng M, Yin N, Li MO
  TITLE     Sestrins function as guanine nucleotide dissociation inhibitors for 
            Rag GTPases to control mTORC1 signaling.
  JOURNAL   Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038.
  PUBMED    25259925
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-25- 
            25"; volume: "159"; issue: "1"; pages: "122-33"
COMMENT     Sequence Label: pcDNA3.1 Flag-Sesn1b
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccgcca
      121 ccatggacta caaagacgat gacgacaaga tgcgcctggc cgccgcgtcg aacgaggcgt
      181 acgcagcctc gctggctgtc tccgagctgc tgagctgcca ccagtgcggc ggggaccgcg
      241 gccaggacga ggaacttgga atcagaatcc ctcggccact aggacacgga ccaagcaggt
      301 tcatcccaga gaaggagatg ctgcaggtgg gcagtgaaga cgcacagatg catgcgttat
      361 ttgcagactc ctttgctgct ttgggtcgtc tggataacat cacattagtg atggttttcc
      421 atccacaata tttagaaagt ttcttaaaga ctcaacacta cctactacaa atggatgggc
      481 cattacccct ccattatcgg cactacattg gaatcatggc ggcagcgagg catcagtgtt
      541 cctacctggt gaatctgcat gtcagtgact ttcttcatgt tggtggggac cccaagtggc
      601 tcaatggttt agagaatgct cctcaaaaac tgcagaactt aggagaactt aacaaagtgt
      661 tagcccatag gccttggctg attaccaaag aacatatcga gggccttctg aaagcagagg
      721 agcacagctg gtctctcgcc gagctggtcc atgccgtggt tctgctcaca cactaccact
      781 ctcttgcctc cttcac
//
