LOCUS       sequence-102520-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient overexpression of Flag-Sesn2 (mouse).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Peng M, Yin N, Li MO
  TITLE     Sestrins function as guanine nucleotide dissociation inhibitors for 
            Rag GTPases to control mTORC1 signaling.
  JOURNAL   Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038.
  PUBMED    25259925
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-25- 
            25"; volume: "159"; issue: "1"; pages: "122-33"
COMMENT     Sequence Label: pcDNA3.1 Flag-Sesn2
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..150
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccgcc
      121 accatggact acaaagacga tgacgacaag atgatcgtag cggactccga gtgccattcc
      181 gagatcaagg gctacctgcc gttcaccagg ggtggtgtgg ccggccccga gacccgagag
      241 gagcatcggg agggccaggc tcggcgaggc tctcgagggc ccagcgcttt cattccagtg
      301 gaagagatcc ttcgggaagg agctgagagc ctggagcagc acctggggct cgaggccctg
      361 atgtcctcag ggcgggtgga caacctggcg gtggtgatgg gtctacaccc ggactacctt
      421 agcagcttct ggcgcctgca ttacctgctg ctgcatacgg atgggcccct ggccagctcc
      481 tggcgccact acattgccat catggctgct gcccgtcacc agtgttctta cctggtgggt
      541 tcccacatga ctgagttcct gcagaccggc ggtgaccccg agtggctgct gggccttcac
      601 cgggcccctg agaagctccg caagctcagc gaggtcaaca agttactggc gcatcggccg
      661 tggctcatca ccaaggagca tatccaggcc ttgctgaaga ctggcgagca cagctggtcc
      721 ctggccgaac tcatccaagc actggtcctg cttacccact gccactctct ggcctccttt
      781 gtg
//
