LOCUS       sequence-102523-       11088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The mouse Dpyd was used to rescue the human DPYD hairpin affect on 
            EMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Shaul YD, Freinkman E, Comb WC, Cantor JR, Tam WL, Thiru P, Kim D, 
            Kanarek N, Pacold ME, Chen WW, Bierie B, Possemato R, Reinhardt F, 
            Weinberg RA, Yaffe MB, Sabatini DM
  TITLE     Dihydropyrimidine accumulation is required for the 
            epithelial-mesenchymal transition.
  JOURNAL   Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.
  PUBMED    25171410
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.07"; journalName: "Cell"; date: "2014-08-28- 
            28"; volume: "158"; issue: "5"; pages: "1094-109"
COMMENT     Sequence Label: pLJM5-I560S-mDPYD
FEATURES             Location/Qualifiers
     source          1..11088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    3499..3616
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     4108..4127
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             5596..5829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5901..6022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5938..5957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5992..6011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6062..6197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6217..6236)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6218..6236)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6246..6263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6246..6262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6255..6277)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6404..6859
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6491..6510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6701..6722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6885..6989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6990..7058,7059..7850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7208..7227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8021..8609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8510..8529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8763..8780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8897..8918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8933..8938,8939..8956,8957..8963)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8971..8987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8976..8998
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8995..9011
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8995..9011
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9030..9050
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9032..9050
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9078..9304
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9305..9485
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9532..9657
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10150..10383
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10568..10612
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     10910..10930
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcagcgctgc catggcgggt gtgctgagca gggacgcgcc ggacatcgag agtatcctgg
      421 ctttaaaccc tcgagtacaa gctcatgcaa ctctgcgttc caccgcagcc aagaaactag
      481 acaagaaaca ctggaaaagg aacacggaca agaactgctt tacctgtgag aagctggaga
      541 gtaattttga tgacatcaag cacacgactc ttggtgagcg aggagctctc cgagaagcag
      601 tgagatgcct gaagtgcgca gatgctccct gccagaagag ctgtcccaca tctcttgaca
      661 ttaaatcatt tatcacaagt atcgccaaca agaactacta tggtgcagct aagctgattt
      721 tttctgataa ccctcttggt cttacttgtg gaatggtttg tccaacatct gacctctgtg
      781 ttggaggatg caacttacat gctgctgaag agggaccaat taatattggt gggctgcagc
      841 agtttgctac tgaggtgttc aaagcaatga acatcccaca gatcagaaac ccatcgctgc
      901 ctcctccgga acatatgccg gaagcctact cagcaaaaat tgcacttttt ggtgcagggc
      961 ctgcgagtat aagctgtgcc tcctttttgg ctcggttggg ctattccaac attactatat
     1021 tcgaaaaaca agaatatgtt ggtggtttaa gcacttctga aatccctcag tttcgactcc
     1081 catatgacgt cgtgaatttt gagattgagc tcatgaagga ccttggtgta aagataattt
     1141 gtggtaaaag cctttccacc gatgaaatga ctcttagctc tttgaaagaa aatggctaca
     1201 gagctgcatt tattggaata ggcttgccag aacccaaaaa ggaccatatt ttccaaggct
     1261 tgacacaagt ccagggattt tacacatcta aagactttct gccacttgtc gccaaaagca
     1321 gtaaaacagg aatgtgcgcc tgtcactctc cattgccatc cataagggga gccgtgattg
     1381 tactcggagc tggagatact gcgtttgact gtgcaacatc cgctctgcgc tgtggagccc
     1441 ttcgcgtgtt catcgtcttc agaaagggct ttgttaatat tcgagctgtt ccagaggaga
     1501 tggagctcgc taaggaagag aaatgtgaat ttttgccttt cctctcgcca cggaaggtta
     1561 tagtcaaaga tggaaagatt gtggcaatgc aatttgttcg aactgagcaa gatgaaaccg
     1621 gaaactgggt ggaagatgaa gagcagacgg tgcggctaaa ggctgatgtg gttattagtg
     1681 cctttggatc tgtcctggag gatcccaaag tgaaagaagc attgagtccc atcaagttta
     1741 acagatgggg tctcccagaa gtaaatccag aaaccatgca aaccagtgaa ccatgggtgt
     1801 ttgcaggtgg tgatgttgtg ggtatggcta acaccacagt ggaatctgtc aacgatggaa
     1861 agcaagcttc atggtacatt cacaagcaca tacaggcaca atatggaacc tcagtgcctt
     1921 ctcagcctac aatgccccta ttttacactc cggttgactt agtggacatc agcgtggaaa
     1981 tggcagggtt gaggtttccc aatccctttg gccttgccag tgcgacacca gccactagca
     2041 caccaatgag tcgaagggcc tttgaagctg gctggggttt tgctctgacc aaaactttct
     2101 ctcttgataa ggacatcgtg acaaacgtct caccaagaat catccggggg acaacttctg
     2161 gccccttgta tggccctgga caaagctcct ttcttaacat tgagctcatc agtgagaaaa
     2221 cagctgcata ttggtgtcac agtgtcaccg aactaaaggc tgacttcccg gacaatatcc
     2281 tgatcgccag catcatgtgc agttacaaca agagtgactg gatggaactc tccaaaatgg
     2341 ctgaggcttc tggagcagat gccctggagt taaatttatc gtgtccacat ggcatggggg
     2401 agagaggaat gggcctggct tgtgggcagg atccagagct ggtgaggaac atctgtcgct
     2461 gggttaggca agctgttcgg gttccatttt ttgccaagtt gaccccaaat gtcactgata
     2521 ttgtaagcat cgcaagagca gcaaaggaag gtggagcaga tggtgtaaca gccaccaaca
     2581 ccgtctcggg tctgatggga ctgaaagctg atggcacacc ctggcctgct gtgggcattg
     2641 gaaggaggac tacatatgga ggagtgtcag gaactgctat caggcccatt gctttgagag
     2701 ctgtgaccgc cattgcccgt gctttgcctg ggtttcctat tttggccaca ggtggaattg
     2761 actcagctga aagtggactt cagtttcttc atagtggtgc ttcagttctt caggtatgca
     2821 gcgctattca gaatcaggac ttcactgtga ttgaagatta ctgcactgga ctcaaagccc
     2881 tgctttatct gaagagcatt gaagagttag cagactggga tggtcagagt ccacccatta
     2941 taagtcatca gaaagggaaa ccagttccac gcgttgctga gctcatggga cagaaactgc
     3001 ccagctttgg accttacctt gaacagcgca agaaaatcat agcagcaagc aaaatcagac
     3061 agaaagatca aaacacagct tgctcacctc tccagagaaa gcactttaac tcgcaaaagc
     3121 ctattcctgc catcaaggat gtaattggaa aatcactgca atacctggga acatttggtg
     3181 agatgagcat catggaacaa gttgtggccc tgatcgatga ggaaatgtgc atcaattgcg
     3241 gcaaatgtta catgacctgt aatgactctg gctaccaggc tatccagttc gatccagaaa
     3301 ctcacctgcc taccgttagc gacacatgta caggctgtac tctctgcctc agcgtctgcc
     3361 ctattatgga ctgtatcagg atggtttcca gggcaacacc ttatcaacca aagagaggcc
     3421 taccattagc cgtgaagccg gtgtgttaag gtaccatgaa ttctcgacct cgagacaaat
     3481 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3541 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3601 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
     3661 cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     3721 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     3781 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     3841 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     3901 aaacggaagc cgcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3961 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     4021 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     4081 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     4141 cgctgttctc ctcttcctca tctccgggcc tttcgacctg catcccgcca ccatgaaaaa
     4201 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     4261 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     4321 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     4381 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     4441 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     4501 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     4561 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     4621 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     4681 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     4741 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     4801 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     4861 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     4921 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     4981 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5041 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5101 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5161 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     5221 gtagatgccg accgaacaag agctgatttc gagaacgcct cagccagcaa ctcgcgcgag
     5281 cctagcaagg caaatgcgag agaacggcct tacgcttggt ggcacagttc tcgtccacag
     5341 ttcgctaagc tcgctcggct gggtcgcggg agggccggtc gcagtgattc aggcccttct
     5401 ggattgtgtt ggtccccagg gcacgattgt catgcccacg cactcgggtg atctgactga
     5461 tcccgcagat tggagatcgc cgcccgtgcc tgccgattgg gtgcagatcc gtcgacctgc
     5521 agccaagctc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5581 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5641 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5881 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6001 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6061 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6121 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6181 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6241 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6301 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6361 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6421 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6481 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6541 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6601 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6661 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6721 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6781 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6841 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6901 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6961 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7201 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7441 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8161 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8701 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8761 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8821 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8881 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8941 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9001 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9061 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     9121 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9181 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9241 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9301 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9361 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9421 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9481 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9541 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9601 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9661 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9721 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9781 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9841 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9901 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9961 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
    10021 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    10081 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    10141 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    10201 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10261 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10321 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10381 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10441 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10501 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10561 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10621 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10681 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10741 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
    10801 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    10861 tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg agggcctatt
    10921 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
    10981 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
    11041 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatca
//
