LOCUS       sequence-102524-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient overexpression of Flag-Sesn3 (mouse).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Peng M, Yin N, Li MO
  TITLE     Sestrins function as guanine nucleotide dissociation inhibitors for 
            Rag GTPases to control mTORC1 signaling.
  JOURNAL   Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038.
  PUBMED    25259925
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-25- 
            25"; volume: "159"; issue: "1"; pages: "122-33"
COMMENT     Sequence Label: pcDNA3.1 Flag-Sesn3
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..150
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccgcc
      121 accatggact acaaagacga tgacgacaag atgaaccgcg gtggcagcag cgcgtcggcc
      181 tcggccaact acctgctctg caccaactgc cggaaagtgc tgcggaagga caaaagaatc
      241 cgagtgtctc aacccctgac aagaggacca agtgccttta ttccagagaa ggaagttgtc
      301 caagcaaata cggcggatga acgaacaaac tttcttgtgg aagagtactc tacatccggt
      361 cgcctggaca acatcacaca ggtgatgagc ttgcacactc agtacctgga atctttcttg
      421 cggagccagt tctacatgct gcgtatggac ggcccccttc ccctcccaga caggcactac
      481 attgccatca tggctgcagc cagacaccag tgttcctacc taataaacat gcatgtggat
      541 gaatttttaa agactggagg gatcgctgag tggctgaatg gcttggaata tgtaccacaa
      601 agactgagaa atcttaatga aattaacaag ctgctggcac accggccctg gctgatcaca
      661 aaagagcaca ttcagaaact tgtcaaaact ggagaaaata attggtccct gcctgagcta
      721 gtgcacgccg tggtcctctt ggctcactat cacgc
//
