LOCUS       sequence-102529-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox inducible lentivirial expression of Flag-Sesn 419A/422A/426A 
            (mouse) mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Peng M, Yin N, Li MO
  TITLE     Sestrins function as guanine nucleotide dissociation inhibitors for 
            Rag GTPases to control mTORC1 signaling.
  JOURNAL   Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038.
  PUBMED    25259925
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-25- 
            25"; volume: "159"; issue: "1"; pages: "122-33"
COMMENT     Sequence Label: pInducer21 Flag-Sesn2 419A/422A/426A
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atagaagaca ccgggaccgt atccagcctc cgcggccccg aactagtcca gtgtggtgga
       61 attctgcaga tatcaacaag tttgtacaaa aaagcaggct accatggact acaaagacga
      121 tgacgacaag atgatcgtag cggactccga gtgccattcc gagatcaagg gctacctgcc
      181 gttcaccagg ggtggtgtgg ccggccccga gacccgagag gagcatcggg agggccaggc
      241 tcggcgaggc tctcgagggc ccagcgcttt cattccagtg gaagagatcc ttcgggaagg
      301 agctgagagc ctggagcagc acctggggct cgaggccctg atgtcctcag ggcgggtgga
      361 caacctggcg gtggtgatgg gtctacaccc ggactacctt agcagcttct ggcgcctgca
      421 ttacctgctg ctgcatacgg atgggcccct ggccagctcc tggcgccact acattgccat
      481 catggctgct gcccgtcacc agtgttctta cctggtgggt tcccacatga ctgagttcct
      541 gcagaccggc ggtgaccccg agtggctgct gggccttcac cgggcccctg agaagctccg
      601 caagctcagc gaggtcaaca agttactggc gcatcggccg tggctcatca ccaaggaaca
      661 tatccaggcc ttgctg
//
