LOCUS       sequence-102530-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing TRP1 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5608_TEF1*-Cre_TRP1
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..282
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             283..957
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 aacgacatta ctatatatat aatataggaa gcatttaata gaacagcatc gtaatatatg
       61 tgtactttgc agttatgacg ccagatggca gtagtggaag atattcttta ttgaaaaata
      121 gcttgtcacc ttacgtacaa tcttgatccg gagcttttct ttttttgccg attaagaatt
      181 aattcggtcg aaaaaagaaa aggagagggc caagagggag ggcattggtg actattgagc
      241 acgtgagtat acgtgattaa gcacacaaag gcagcttgga gtatgtctgt tattaatttc
      301 acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa
      361 tgtgctctag attccgatgc tgacttgctg ggtattatat gtgtgcccaa tagaaagaga
      421 acaattgacc cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt
      481 tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg
      541 gctctggtca atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcaa
      601 gaataccaag agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc
      661 aacatactac tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca
      721 gaagcaggtg ggacaggtga acttttggat tggaactcga tttctgactg ggttggaagg
      781 caagagagcc ccgaaagctt acattttatg ttagctggtg gactgacgcc agaaaatgtt
      841 ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg taagcggagg tgtggagaca
      901 aatggtgtaa aagactctaa caaaatagca aatttcgtca aaaatgctaa gaaatag
//
