LOCUS       sequence-102531-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox inducible lentivirial expression of Flag-Sesn 419A/422A/426A 
            (mouse) mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Peng M, Yin N, Li MO
  TITLE     Sestrins function as guanine nucleotide dissociation inhibitors for 
            Rag GTPases to control mTORC1 signaling.
  JOURNAL   Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038.
  PUBMED    25259925
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-25- 
            25"; volume: "159"; issue: "1"; pages: "122-33"
COMMENT     Sequence Label: pInducer21 Flag-Sesn2 419A/422A/426A
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..36)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcattactaa gcgtagtctg ggacgtcgta tgggtattcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggttcaggtc
      121 atgtagcggg tgatggcgcg cagggcatag agcagggccg cttgcatgcg ggcttcaagg
      181 agcagcaagt tcacatgaac cttctctgag tggcggaagt gcctccaaaa gaggttgtac
      241 atcctacggg tcgtcttctc agggtagcag gccaccgtcg cgatatagat tgcgaggttc
      301 gcttccagga gctggtttac ctcgccgtaa tcatagtcat cgtatctgat gccaaagacg
      361 cagtggatgt agttccagat ggccctgcgg agcatggagg tgtccacgcc actgtgcatg
      421 gcgatggtgt tataggtgag gctgcaggct acctggaact tctcatccag cagctgccct
      481 ccctcagggt agagcctctg gatcagcgag tagccatggt cttcccaggt ataatcctgg
      541 gcacggaagg ttgggggcgc ctgagtccct cgacgagtga agtcttcata tccaaatgcg
      601 gggtcctcca caaagcatag gatgtctggg tgtggagaag gctccaggat atccgctgag
      661 ggggtcacca gcaggctttc tgacttctcc agctc
//
