LOCUS       sequence-102552-        9240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Expression Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: pcw107
FEATURES             Location/Qualifiers
     source          1..9240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..516
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     435..453
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    537..661
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(686..691,692..709,710..716)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             770..1429
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(836..855)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1771..2076
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1955..1974
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2117..2241)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2273..2861
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2326..2346)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2744..2755)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2933..3166
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3244..3365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3281..3300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3335..3354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3405..3540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3560..3579)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3561..3579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3589..3606)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3589..3605)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3598..3620)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3747..4202
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3834..3853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4044..4065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4228..4332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4333..4401,4402..5193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4551..4570)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5364..5952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5853..5872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6106..6123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6240..6261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6276..6281,6282..6299,6300..6306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6314..6330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6319..6341
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6338..6354
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6338..6354
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6373..6393
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6375..6393
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6421..6647
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6648..6828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6875..7000
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7493..7726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7911..7955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8186..8396
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8208..8404
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8255..8390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8436..9038
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8436..8455)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8932..8952
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9081..9198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
       61 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      121 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      181 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      241 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      301 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      361 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      421 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      481 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatcgat ggatcaacaa
      541 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      601 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      661 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      721 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      781 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      841 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      901 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      961 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     1021 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1081 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1141 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1201 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1261 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1321 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1381 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc
     1441 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     1501 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     1561 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     1621 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     1681 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     1741 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     1801 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     1861 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     1921 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     1981 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     2041 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     2101 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     2161 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     2221 agctttcttg tacaaagtgg ttaccggtta gtaatgaacg cgttaagtcg acaatcaacc
     2281 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2341 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2401 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2461 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2521 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2581 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2641 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     2701 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     2761 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     2821 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     2881 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     2941 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     3181 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3241 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3301 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3361 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     3421 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3481 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3541 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     3601 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3661 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3721 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3781 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3901 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3961 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4021 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4081 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4141 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4201 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4261 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4321 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4381 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4441 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4501 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4561 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4621 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4681 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4741 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4801 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4861 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4921 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4981 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5041 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5101 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5161 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5221 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5281 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5341 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5401 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5461 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5521 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5581 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5641 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5701 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5761 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5821 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5881 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5941 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6001 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6061 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6121 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6181 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6241 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6301 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6361 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     6421 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     6481 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     6541 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     6601 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     6661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6841 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6901 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6961 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7021 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     7081 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7141 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7201 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7261 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7321 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7381 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7441 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7501 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7561 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7621 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7681 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7741 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7801 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7861 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7921 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7981 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8041 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8101 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     8161 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     8221 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8281 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8341 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8401 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     8461 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     8521 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     8581 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     8641 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     8701 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     8761 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     8821 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     8881 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8941 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     9001 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     9061 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9121 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9181 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
//
