LOCUS       sequence-102573-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses horse holocytochrome c in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Patel CN, Lind MC, Pielak GJ
  TITLE     Characterization of horse cytochrome c expressed in Escherichia 
            coli.
  JOURNAL   Protein Expr Purif. 2001 Jul;22(2):220-4.
  PUBMED    11437597
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2001-07"; volume: "22(2)"; pages: "220-4"
COMMENT     Sequence Label: pBTR(hCc)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             25..47
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ataactaact aatctagaaa taattttgtt taactttaag aaggagatat atccatgggc
       61 gacgtggaaa aaggcaaaaa gatcttcgtg cagaaatgcg gctgccacac ggtggaaaaa
      121 ggcggcaaac acaaaaccgg tcccaacctg cacggcctgt tcggccgcaa aacgggccag
      181 gcgccgggct tcacgtacac ggacgcgaac aaaaacaaag gcatcacgtg gaaagaagaa
      241 acgctgatgg aatacctcga gaacccgaaa aaatacatcc cgggcacgaa aatgatcttc
      301 gcgggcatca aaaaaaaaac ggaacgcgaa gacctgatcg cgtacctgaa aaaggcgacg
      361 aacgaatgat aaggtaccaa gatggccttt ggtgggttga agaaggaaaa agacagaaac
      421 gacttaatta cctacttgaa aaaagccact gagtaaacag gccccttttc ctttgtcgat
      481 cggatccggc caagctagct tttcagtaat tatcttttta gtaagctagc taagttttta
      541 cacttagtta aattgctaac tttatagatt acaaaactta ggagggtatc gatactatga
      601 attcacaaaa aatgggttgg ttttgggcag atcaaaaaac tacgggcaaa gatattggtg
      661 gggcagcagt atcatccatg tcagggtgcc cagtcatgca cgagtcgtcg tcgtcgtcgc
      721 caccatcctc tgagtgcccc gttatgcagg gagataacga tagaataaac ccgctgaaca
      781 atatgccgga gttggcagca tccaaacagc ctggcc
//
