LOCUS       sequence-102578-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that allows an arabinose-inducible expression of the I-SceI 
            homing endonuclease - for the en passant (two-step Red-mediated) 
            mutagenesis .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Tischer BK, von Einem J, Kaufer B, Osterrieder N
  TITLE     Two-step red-mediated recombination for versatile high-efficiency 
            markerless DNA manipulation in Escherichia coli.
  JOURNAL   Biotechniques. 2006 Feb;40(2):191-7.
  PUBMED    16526409
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2006-02"; volume: "40(2)"; pages: "191-7"
COMMENT     Sequence Label: pBAD-I-SceI
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
       61 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
      121 tctctactgt ttctccatac ccgttttttt gggctagcag gagggtacct atatgcatat
      181 gaaaaacatc aaaaaaaacc aggtaatgaa cctgggtccg aactctaaac tgctgaaaga
      241 atacaaatcc cagctgatcg aactgaacat cgaacagttc gaagcaggta tcggtctgat
      301 cctgggtgat gcttacatcc gttctcgtga tgaaggtaaa acctactgta tgcagttcga
      361 gtggaaaaac aaagcataca tggaccacgt atgtctgctg tacgatcagt gggtactgtc
      421 cccgccgcac aaaaaagaac gtgttaacca cctgggtaac ctggtaatca cctggggcgc
      481 ccagactttc aaacaccaag ctttcaacaa actggctaac ctgttcatcg ttaacaacaa
      541 aaaaaccatc ccgaacaacc tggttgaaaa ctacctgacc ccgatgtctc tggcatactg
      601 gttcatggat gatggtggta aatgggatta caacaaaaac tctaccaaca aatcgatcgt
      661 actgaacacc cagtctttca ctttcgaaga agtagaatac ctggttaagg gtctgcgtaa
      721 caaattccaa ctgaactgtt acgtaaaaat caacaaaaac aaaccgatca tctacatcga
      781 ttctatgtct tacctgatct tctacaacct gatcaaaccg tacctgat
//
