LOCUS       sequence-102579-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that allows an arabinose-inducible expression of the I-SceI 
            homing endonuclease - for the en passant (two-step Red-mediated) 
            mutagenesis .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Tischer BK, von Einem J, Kaufer B, Osterrieder N
  TITLE     Two-step red-mediated recombination for versatile high-efficiency 
            markerless DNA manipulation in Escherichia coli.
  JOURNAL   Biotechniques. 2006 Feb;40(2):191-7.
  PUBMED    16526409
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2006-02"; volume: "40(2)"; pages: "191-7"
COMMENT     Sequence Label: pBAD-I-SceI
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     184..201
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg
       61 ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca
      121 ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc gcttctgcgt
      181 tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca gccaagcttg
      241 catgcctgca gacgtcgggc ccttatttca ggaaagtttc ggaggagata gtgttcggca
      301 gtttgtacat catctgcggg atcaggtacg gtttgatcag gttgtagaag atcaggtaag
      361 acatagaatc gatgtagatg atcggtttgt ttttgttgat ttttacgtaa cagttcagtt
      421 ggaatttgtt acgcagaccc ttaaccaggt attctacttc ttcgaaagtg aaagactggg
      481 tgttcagtac gatcgatttg ttggtagagt ttttgttgta atcccattta ccaccatcat
      541 ccatgaacca gtatgccaga gacatcgggg tcaggtagtt ttcaaccagg ttgttcggga
      601 tggttttttt gttgttaacg atgaacaggt tagccagttt gttgaaagct tggtgtttga
      661 aagtctgggc gccccaggtg attaccaggt tacccaggtg gttaacacgt tcttttttgt
      721 gcggcgggga cagtacccac tgatcgtaca gcagacatac gtggtccatg tatgctttgt
      781 ttttccactc gaactgcata cagta
//
