LOCUS       sequence-102581-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for en passant (two-step Red-mediated) mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Tischer BK, von Einem J, Kaufer B, Osterrieder N
  TITLE     Two-step red-mediated recombination for versatile high-efficiency 
            markerless DNA manipulation in Escherichia coli.
  JOURNAL   Biotechniques. 2006 Feb;40(2):191-7.
  PUBMED    16526409
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2006-02"; volume: "40(2)"; pages: "191-7"
COMMENT     Sequence Label: pEPkan-S
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..83
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(214..233)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(298..317)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatccg
       61 attacaagga tgacgacgat aagtagggat aacagggtaa tcgatttatt caacaaagcc
      121 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
      181 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
      241 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      301 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
      361 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      421 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
      481 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
      541 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      601 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
      661 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
      721 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
      781 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aacctta
//
