LOCUS       sequence-102584-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for en passant (two-step Red-mediated) mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Tischer BK, von Einem J, Kaufer B, Osterrieder N
  TITLE     Two-step red-mediated recombination for versatile high-efficiency 
            markerless DNA manipulation in Escherichia coli.
  JOURNAL   Biotechniques. 2006 Feb;40(2):191-7.
  PUBMED    16526409
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2006-02"; volume: "40(2)"; pages: "191-7"
COMMENT     Sequence Label: pEPkan-S
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(122..141)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(123..141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(186..389)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(215..235)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        390..769
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca agcttgggtc
      121 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
      181 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      241 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      301 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      361 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      421 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
      481 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
      541 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
      601 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
      661 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
      721 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat
      781 ct
//
