LOCUS       sequence-102593-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of GFP-Sec61 beta .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Zuleger N, Kelly DA, Richardson AC, Kerr AR, Goldberg MW, Goryachev 
            AB, Schirmer EC
  TITLE     System analysis shows distinct mechanisms and common principles of 
            nuclear envelope protein dynamics.
  JOURNAL   J Cell Biol. 2011 Apr 4;193(1):109-23. doi: 10.1083/jcb.201009068. 
            Epub 2011 Mar 28.
  PUBMED    21444689
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201009068"; journalName: "J Cell Biol"; date: 
            "2011-04-4- 4"; volume: "193"; issue: "1"; pages: "109-23"
COMMENT     Sequence Label: Sec61 beta pAcGFP-C1 (802)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..345
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     polyA_signal    506..627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(543..562)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     597..616
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(771..792)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccgccgcca tcacccacgg catggatgag ctgtacaagt ccggactcag atctatgcct
       61 ggtccgaccc ccagtggcac taacgtggga tcctcagggc gctctcccag caaagcagtg
      121 gccgcccggg cggcgggatc cactgtccgg cagaggaaaa atgccagctg tgggacaagg
      181 agtgcaggcc gcacaacctc ggcaggcacc ggggggatgt ggcgattcta cacagaagat
      241 tcacctgggc tcaaagttgg ccctgttcca gtattggtta tgagtcttct gttcatcgct
      301 tctgtattta tgttgcacat ttggggcaag tacactcgtt cgtaggaatt ctgcagtcga
      361 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
      421 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      481 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      601 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
      661 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      721 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      781 acaagagtcc actattaaag aacgtggact ccaacgtcaa
//
