LOCUS       sequence-102601-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a single-chain TALEN targeting ABL1 site in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Sun N, Zhao H
  TITLE     A single-chain TALEN architecture for genome engineering.
  JOURNAL   Mol Biosyst. 2014 Mar 4;10(3):446-53. doi: 10.1039/c3mb70412b. Epub 
            2013 Dec 16.
  PUBMED    24336919
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70412b"; journalName: "Mol Biosyst"; date: "2014-03-4- 
            4"; volume: "10"; issue: "3"; pages: "446-53"
COMMENT     Sequence Label: pCMVJ-scTALEN-ABL1-R
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(206..793)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgggatcc tctagagtcg acttaaaagt ttatctcgcc gttattaaat ttccgtctca
      241 cttcctctaa ggttaatgtg ccggctttaa tcatttctcc accaattaaa agctcttcta
      301 cactaagaac agctccatta caattagtga tatgatttaa tcgtgtaagc tgagctttgt
      361 agtttccttt aaagtgacca ctcacaaata aaaacttaaa ttccgttaca gaagatggat
      421 agactttcca ccattcatta gggttgatat gtttgtttcg tgtttgattt tcttcgacat
      481 atcgttgcat ttcatctgct tggccaattg gcagattata acctccgcta taagctttag
      541 tatccacgat cacaccgtaa tcaataggag atccgacagt ataaattgct ccgtccggtt
      601 tccttgatcc acccaaatgt ttacctctat atccataaac tttcataaaa aattccatta
      661 ccttcatttc aagaattcta tcctgagtgg aatttctggc aatttcaatt aattcaatat
      721 attcatgagg cacatatttc aatttatgac gaagttcaga tttcttctcc tccagttcac
      781 ttttgactag ttgactcgag ccgcttcccg acccagatcc cataaacacg gagaccttct
      841 ttctgaccat cgcagtggtc tggtccgtgt aagcagatcc tgtaaccgga atgcccagct
      901 tgatggattt cgcgaggggc gctaccccat tgatagatcc gatggtccag agagtcggct
      961 ggatggagtt cgtgatgtgc gtcgtcaagg gtatagatcc tgcagacata tatatcttcg
     1021 gaacaacatt cctggggttc gtggtccggg ttatagatcc tgagccgcta ccggatccaa
//
